bestall

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Imagine the possibility of having and using a frequency that allows you to direct the tempo of a
certain thought or emotion to offset external stresses. This unique tool and method is unlike any
other on the planet and is the result of over fifteen years of dedicated research and development.
Highly precise acoustic models of parts of the conscious mind itself, something our body-mind is
already familiar with on a primal level, it already knows these sounds intuitively for these are the
sounds it naturally recognizes from nature and that it, itself generates and uses!

A NEW EXPERIMENTAL AND HIGHLY EFFECTIVE FORM OF BEHAVIOUR

AND CONSCIOUSNESS ENTRAINMENT.

The internal rhythmic tempo associated with various internal states, emotional, mental, physical

are mapped. These psycho-somatic patterns are captured as hyper-dimensional geometric forms.

The complex numeric series representing the movement of this object through space-time is translated

into sound.

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These items are MP3 digital audio tracks which can be played via normal audio players or through ‘silent delivery emitters’

ACCESSING YOUR FILES ;
The files you order will be accessible via download links sent to your email address from "Tradebit", check your email inbox or Trash folder to locate this email. If you need further instructions see this video to step you through the download process ;
http://www.youtube.com/watch?v=0ZVwR_3aKRk

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USING YOUR FREQUENCIES ( HOW TO ) ;
FIRST, ensure you have watched this video in entirety
http://www.youtube.com/watch?v=gI_z-PgwMhs

For most frequency kits, usage is simple. Place all of them on your player and play only the first 30 seconds of each ten minute track, skip through all nine playing only the first 30 seconds, note out of the nine which tracks effect you the most, likely there will be between 2 to 4 tracks that really stand out, you will feel this. Next, select only those tracks and loop these together for your session(s). Keep in mind that your body-mind will change its preference over time so you will need to do this through out the quarters of a given lunar month.

MORE USAGE INFO :
http://www.soundofstars.org/howtouse.htm

ADDITIONAL INSTRUCTIONS FOR USE;
Grab a notepad and pen and keep these close to you. Once you have downloaded this track, set your volume fairly loud but within comfort levels. Loop the track and allow it to play for a minimum of 20 minutes. Close your eyes and sit in a comfortable position, breathe deeply in through your nose and out through your mouth. Relax.

Pay attention to what you are experiencing. Once the experience is over, grab your note pad and pen and write down the details of what you felt, saw, thought, experienced. Report these effects to our group(s).

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Professionals, including scientists & engineers have already attested to the validity of this work, even lab tests have been done, records taken.

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CANCER KILLED BY STAR LIGHT & FREQUENCIES ?
(REMEMBER YOUR BODY IS MOSTLY WATER ! )

Now astronomers think they might have a new technique for doing so, with an added bonus: it could also lead to a potential new cancer radiation treatment capable of precisely targeting tumors without damaging the surrounding healthy tissue. Anil Pradham, an astronomer at Ohio State University described the research at the International Symposium on Molecular Spectroscopy in June.

http://news.discovery.com/space/for-new-cancer-therapies-look-to-the-stars-110726.html

7 Jul 2008 ... These ideas were the thoughts of some "non-scientists" until French physicist and musician, Joel Sternheimer, discovered the mechanism for ...
biogenesislab.blogspot.com/.../joel-sternheimer-earthpulse-press.html - Cached - Similar

The Canadian researcher M.A. Persinger discusses possible mechanisms for how an extremely weak extra low frequency (VLF) EM field might affect bioregulatory ...
www.proactivehealthnet.com/healthBB/archive/index.php?t-1851... - Cached

Scientists and doctors Robert Becker, John Ott, Walter Russell, Hans Nieper, M.D., Edward Russell, Abram Ber, Albert Einstein, William Tiller, Nikola Tesla, Edgar Cayce, Edward Bach, Gabriel Cousins, M.D., David Tansley, Marcel Vogel, Semyon and Valentina Kirlian, John Fetzer, Wilhem Reich, Christopher Hills, realise that the universe works electrically, among others.

"Living creatures consist of invisible electro-vibratory fields that not only form and develop what we recognize as our physical bodies, but also control the creation of new cells. These new cells replace those that are injured, worn out, or die from disease, impaired nerve impulse or environmental poisoning". Dr Cyril Smith, Nobel Prize winner in 1925 for cell electrical current research.

Soviet scientist, Dr. A.S. Presman (late 1960’s), discovered cells constantly communicate with each other through coded energy patterns. He studied a broad range of effects of the electromagnetic spectrum on living systems. He found that electromagnetic radiation can affect the spatial orientation of animals, modulate the rhythm of their physiological processes, and effect vital living processes adversely. He concluded electromagnetic fields serve as conveyers of information from the environment to the organism, within the organism, and among organisms. His data shows increases in the sensitivity to electromagnetic fields. The more complex the organism, the greater the sensitivity. Since man is the most complex, we are the most affected.

Georges Lakhovsky, 1925, stated, "the nucleus of a living cell may be compared to an electrical oscillating circuit. This nucleus consists of tubular filaments, chromosomes and mitochondria, made up of insulating material and filled with a conducting fluid containing all the mineral salts found in sea-water. These filaments are thus comparable to oscillating circuits endowed with capacity and self-inductance and therefore capable of oscillating according to a specific frequency." The Secret of Life, page 7.

Dr. Hans Jenny, 1972,was convinced that biological evolution was a result of vibrations, and that their nature determined the ultimate outcome. He speculated that every cell had its own frequency and that a number of cells with the same frequency created a new frequency which was in harmony with the original, which in its turn possibly formed an organ that also created a new frequency in harmony with the two preceding ones. Jenny was saying that the key to understanding how we can heal the body with the help of tones lies in our understanding of how different frequencies influence genes, cells and various structures in the body.

"This electrical system," says Nordenstrom (1980’s), "works to balance the activity of internal organs and in the case of injuries, represents the very foundation of the healing process." The electrical currents of the body are as critical to the well-being of the human body as the flow of blood. Disturbances in this electrical network may be involved in the development of cancer and other diseases.

Dr. Nancy Wertheimer (early 2000’s) Ph.D. epidemiologist who has been studying Electromagnetic Fields (EMF’s) for 20 years, has been looking at the epidemiological data in a different way — she is trying to associate EMF levels with health rather than disease. The level she is coming up with is a cut off of 1 milliGauss (mG). Russian researchers claim that 1/1000ths of a mG should be the standard.

The Bio-Electric Body believes that there are several stages of health between "optimum wellness", "degenerative disease" and "Cancer". Thus, we maintain our own living and sleeping quarters at 0.5mG and below.

Carlo Rubbia, (1984 Nobel Prize), "energy is superior to matter and determines its structure".

Cell researcher Bruce Lipton (current), PhD., "cells are 100 times more sensitive to electromagnetic information (signals) than to chemical information".

Dr. Valerie Hunt (1970’s on) has devoted the last 25 years to measuring and mapping human bio-energy. She is the first researcher to make objective, repeatable measurements of the full spectrum of the field surrounding the human body. Her most important readings occur in much higher vibrational ranges than those pertaining to muscle tension - with frequencies in the megahertz range and above. She reports that the energetic emanations of the body show anti-coherent changes before actual disease conditions can be diagnosed.

"The energy field starts it all". Dr. Burr (1940’s) discovered that all living things - from men to mice, from trees to seeds - are moulded and controlled by electro-dynamic fields, which could be measured and mapped with standard voltmeters. These "fields of life," or L-fields, are the basic blueprints of all life on this planet.

Nobel Prize winner Lehninger (1979) mentions in his textbook that some reactions in the living cell happen quite a lot faster than what corresponds to 37° temperature. The explanation seems to be that the body purposely directs chemical reactions by means of electromagnetic vibrations (biophotons).

Prof Fritze-Albert Popp (1975)proved that emission from the cells is not a result of DNA propagation, but the electromagnetic frequency directing the division of cells. Conclusive experimental evidence has been published to show that DNA is the source of these electromagnetic frequency and there is evidence to support the idea that electromagnetic frequency are responsible for triggering biochemical reactions in living cells.

Reba Goodman, Ph.D. Professor Emeritus Clinical Pathology; Special Lecturer in Pathology 2001. The conventional stress-activated signal transduction pathways may not be the only mechanisms for extracellular signalling to the nucleus. Magnetic field-stimulated transcription in cell-free preparations implies that membrane interaction is not an essential element in transmitting the signal to the nucleus. Since magnetic fields penetrate cells and are not limited to interactions with the membrane there may be a direct effect of magnetic fields on DNA through interaction with conducting electrons in the stacked bases of the DNA. Electromagn Biol Med. 2008 ;27 (1):3-23 18327711 (P,S,E,B). Protein and DNA reactions stimulated by electromagnetic fields.

Martin Blank (1970’s on). Department of Physiology and Cellular Biophysics, Columbia University, New York, New York, USA. Studies with electric and magnetic fields in the extremely low-frequency range have shown that weak fields can cause charge movement. It appears likely that weak EMF can control and amplify biological processes through their effects on charge distribution.

Jacques Benveniste (1985), French immunologist, proposed that cells communicate via electromagnetic signals:

Fig A The 3D structure of an antigen (or an agonist) matches the 3D structure of the antibody (or the receptor, respectively). This physical contact is what induces the cell function.

Fig B The agonist molecule carries and emits an EM signal which co-resonates with the receptors molecules, thus activating it, and inducing the cell function.

The "Electromagnetic Signals" Theory Using various experimental protocols we are able to activate specific cell functions with the corresponding low frequency (<20 kHz) electromagnetic waves. This prompted us to hypothesize that the molecular signal is composed of such low frequency waves and that the ligand co-resonates with the receptor pretty much as the tuning of a radio device.

It is important to remark that these concepts do not violate any current biological or physical basic principle. It is well-documented that:

2) A complex set of high frequency waves can produce low frequencies according to the "beat frequency" phenomenon;

3) All biological interactions occur in water, since, on the average, there are ten thousand molecules of water per molecule of protein.

Quantum electro-dynamics calls for the existence of long range electromagnetic fields that can be transmitted by large - hundreds of angstroms - coherent domains present in water (adapted from E. Del Giudice & E. Preparata, 1994, Journal of Biological Physics, vol. 20, p. 105). Such long range EM fields would be capable of transmitting the EM message coming from molecules, thus generating a long distance specific attraction between two molecules with matching spectra, excluding non-resonating, unwanted random events. The field resulting from the aggregation of the two co-resonating molecules would obviously exhibit a different frequency which would then co-resonate with the next molecule or cluster of molecules which intervene in the next step of the biochemical reaction, and so forth and so on... The fact that small changes in the spectrum of a molecule (e.g. induced by a tiny structural change) would profoundly alter its resonating characteristics, would explain how minute changes (e.g. phosphorylation, replacement of an ion by a similar one, switching of two peptides...) radically modify the molecular tertiary structure and function.

Proof positive - Homeopathy works, but dr jacques isn't around to hear his work vindicated

It is tragic that it has taken 16 years to vindicate the controversial discoveries of Dr Jacques Benveniste - and in the very week that he has died.

Dr Benveniste scientifically proved the basis of homeopathy in a series of experiments with water, which were published in Nature in 1988. Thereafter the medical establishment pilloried him, research funding was taken away, and his very promising career in science was ended. Television magician James Randi visited Benveniste's laboratories but, not surprisingly perhaps, was unable to verify his discovery.
But scientists, rather than celebrities, have finally proved his findings. Four independent laboratories, in Ireland, Italy, France and the Netherlands, have discovered that effects can occur below the level at which any molecule of the substance is present.
Sadly, their findings were published in the week that Dr Benveniste died following heart surgery.
(Source: Inflammation Research, 2004; 53: 181-8).
* The work of Dr Jacques Benveniste, and his struggle against the prevailing establishment, iss outlined in Lynne McTaggart's inspiring book The Field.

http://www.google.ca/search?hl=en&q=%2B%22jacques+benveniste%22+%2B%22cell+%22&meta=&aq=f&aqi=&aql=&oq=&gs_rfai=

The phenomenon of how information is transmitted within living systems was and is being extensively studied by a bio-physicist named Fritz Albert Popp. He discovered that photons provided the vehicle for which information was transmitted. Photons are light particles without mass. They transmit information within a cell and between cells. He showed that DNA of living cells stores and releases photons. He called this "biophotonic emission". The intensity is about 10 (18th) times lower than regular daylight. To study this phenomenon he developed an instrument called a photon multiplier which could detect the glow of a firefly 10 miles away. DNA uses a variety of frequencies as an information tool suggesting a feedback system of perfect communication through waves which encode and transfer information.

Another fascinating characteristic of photons is their coherence. In a healthy state the emission is more coherent than anything that man has ever developed. Quantum coherence means that subatomic particles are able to co-operate. These waves know about each other and are highly interlinked by bands of electromagnetic fields. They can communicate with each other. It is analogous to an orchestra where all photons are playing together but as individual instruments that are able to carry on playing individual parts. Therefore, biophotonic emission is a perfect communication system that transfers information to many cells across the body and to other bodies.

Another important characteristic of biophotons is that they follow biological rhythms (i.e., daily, weekly, monthly and annually). In healthy individuals the biophotons are extremely coherent and in rhythm with the world. In seriously ill people (i.e., cancer) they have lost their natural rhythm and coherence. The lines of communication were scrambled and they lost their connection with the world. In effect their light was going out.

Interestingly, Popp found in people with multiple sclerosis, that the opposite was true. They were in a state of too much order. They were taking in too much light, inhibiting the ability of the cells to do their job. Too much cooperative harmony prevented flexibility and individuality. In reality, they were drowning in their light.

In a stressed state, the rate of biophotonic emissions went up. This is a defense mechanism to try and return to homeostasis. Therefore biophotonic emission is a sort of correction mechanism in living systems.

Studying food, Popp found that the healthiest food has the lowest and most coherent intensity of light.

The final important principle looks at the actual intensity of the emission. William Ross Adey, while experimenting with cerebral cells in chicks, found they only responded to a certain frequency (10 Hz). Also, he found that the amplitude must lie within a specific (very low) range. This is called Adey’s window, and it is a limited range in which a biological system is able to respond to electromagnetic signals carrying information. Interestingly, this range falls within the same range in which Homeopathic signals are measured.

Popp found while studying Homeopathic remedies that they are an example of photon sucking. Homeopathic remedies are resonance absorbers. If a non-physiological frequency produces certain symptoms, a high dilution of a substance which could produce the same symptoms would still carry these oscillations. Like a tuning fork in resonance, a suitable homeopathic solution might attract and then absorb the non-physiological oscillations, allowing the body to return to normal.

ALL BIOCHEMICAL REACTIONS IN LIVING ORGANISMS ARE OPERATED AND REGULATED BY LOW ELECTROMAGNETIC FREQUENCIES.

The implications of this statement are numerous and extremely important, not the least of which is that the biophysical level of an organism controls its biochemistry. For example, changing and adjusting the biochemistry of the human body can easily be accomplished with medications, supplements, diet, and even exercise, but this is only dealing with a disease state at a symptomatic level. However, affecting change at the biophysical level where cellular communication can be normalized will result in a balance of both the biophysical and biochemical levels. And how does one affect this type of change? Based on what we’ve learned from the research of Benveniste, Popp, and Adey, the answer is digital homeopathy.

Abstract FASEB Journal, 1999, vol. 13, p. A852
SPECIFIC REMOTE DETECTION OF BACTERIA USING AN ELECTROMAGNETIC / DIGITAL PROCEDURE

J. Benveniste, L. Kahhak, and D. Guillonnet. Digital Biology Laboratory, F-92140 Clamart.
Previous studies suggest that the electromagnetic molecular signal (EMS) can be digitally recorded and replayed [1-4]. We electronically captured, digitized, and transmitted the specific EMS of bacteria to a biological system sensitive to this EMS. First we digitally recorded EMS from E.coli K1, staphylococcus (3.5 million/ml) or saline. Latex particles, sensitized by a K1 antibody (ab), aggregate to E.coli K1 (kit, Pasteur Diagnostics). We then set up the ab-latex and E.coli antigen (ag) concentrations so as to obtain minimal aggregation. Next, we applied the E.coli or control recorded EMS for 2 min to ab-latex and ag which we subsequently mixed. After allowing the mixture to migrate for 13 min at 37°C in a capillary, we examined it under a microscope. A CCD camera generated 3 pictures that were analyzed using a dedicated software. E.coli K1 EMS induced the formation of larger aggregates than that of Staphylococcus or saline. The magnitude of aggregation varied from day to day. Yet, by comparing E.coli data from controls of the same day, we seldom failed to detect E.coli in hundreds of experiments (mean of differences: 150 %). Thus, we are clearly able to identify a bacteria by playing its EMS signal. The latter is specific, since the effect of staphylococcus EMS on E.coli ag/ab reaction was close to that of saline. Since EMS travel a long distance in the form of a ".wav" file, it should become possible to detect any immunogenic substance from a remote location.

[1] Davenas et al., Eur. J. Pharmacol., 1987, 135:313; [2] Davenas et al., Nature, 1988, 333:816; [3] Aïssa et al., J. Immunol., 1993, 150:A146; [4] Benveniste et al., J. Allergy Clin. Immunol., 1997, 99:S175. Supported by DigiBio S.A.

Abstract
MODULATION OF HUMAN NEUTROPHIL ACTIVATION BY "ELECTRONIC" PHORBOL MYRISTATE ACETATE (PMA).
Yolène Thomas, Hedi Litime and ,Jacques Benveniste. CNRS URA 1442, 60206 Compiègne and Digital Biology, laboratory, 32 rue des Carnets, 92140 Clamart, France.
The molecular signal of PMA can be electronically transmitted and modulate neutrophil reactive oxygen metabolite (ROM) production as if PMA itself was added to the cells [1]. Experiments reported here were done blind or open at INSERM U 332, ICGM, Paris. Transmission was performed via an especially designed amplifier connected to two coils [2]. The source tube containing PMA (1 µM) or vehicle was placed on the input coil at RT, and target cells (1 million per ml) on the output coil ait 37° C in a humidified incubator. The amplifier was then turned on for 15 min transmission time and cells were then incubated for up to 45 min before measurement of cytochrome c reduction. Three or four amplifiers were used simultaneously for each experiment. An increase in ROM production was observed immediately after PMA transmission: (over vehicle transmission), 56.6 +- 15.5 %, mean +- SEM, and up to 60 min: 38.7 +- 14.8, n = 9. Peak PMA transmission effect varied among individuals. Transmission of 4alpha-phorbol 12,13-dibutyrate, an inactive PMA analogue, did not stimulate neutrophil ROM production. The effect of PMA transmission was inhibited when 1) the amplifier was switched off: 42 ± 8 % vs -1.8 ±1.4 %, n = 6, and 2) the selective protein kinase C inhibitor GF109203X was added to the cells (8 µM) before PMA transmission.
These results, together with those previously reported, suggest that molecules emit signals than can be transferred to cells by artificial physical means in a manner that seems specific to the source molecules.
[1] Thomas et al., FASEB J. 1995, 9:A227; [2] Benveniste et al., FASEB J. 1994, 8: A398. Supported in part by Ministère de l'Environnement, Bouygues SA, SAUR, and Association Science Innovante.

Abstract
DIGITAL RECORDING/TRANSMISSION OF THE CHOLINERGIC SIGNAL.
J. Benveniste, P. Jurgens and J. Aissa. INSERM U 200 and Digital Biology 'Laboratory, 32 rue des Carnets, 92140 Clamart, France.

Previous studies suggest that the biological activity of agonists can be transferred to water by electromagnetic means [1-7]. Since July 1995, in keeping with these results, we have digitized, recorded, and 'replayed' to water the activity of acetylcholine (ACh) or water (W) as control. ACh and W were recorded (16 bits, 22 KHz), for 1-5 sec, via an especially designed transducer, on the hard disk of a computer equipped with a Sound Blaster 16 card. Files were digitally amplified and the signal of digitally recorded ACh or W was replayed for 15 min, via the transducer, to 15 ml, W-containing plastic tubes. W thus exposed (dACh, dW), was then perfused to isolated guinea-pig hearts. In 13 open experiments, coronary flow variations were (%, mean +- SEM, nb of samples): W+dW(not stat. diff.), 3.3 +- 0.2, 20; dACh, 16.2 +- 1.0, 33, p = 4.1 e- 10 vs W+dW; ACh (0.1 µM), 23.4 +- 2.8, 12, P = 5 e-3 vs dACh. In 25 blind experiments: W+dW, 3.6 +- 0.3, 61; dACh, 20.4 +- 1.3, 58, p = 1 e- 16 vs W+dW; ACh (0.1 pM), 28.1 +- 2.3, 24, p = 3 e-3 vs dACh. Atropine inhibited the effects of both ACh and dACh. Moreover, we have recently transferred specific digital signals via telephone lines.

These results indicate that the molecular signal is composed of waveforms in the 0-22 Khz range. They open the way to purely digital procedures for the analysis, modification and transmission of molecular activity.

[1] Aissa et al., FASEB J. 1993, 7:A602; [2] Benveniste et al., FASEB J. 1994, 8: A398; [3] Aissa et al., J. Immunol. 1993, 150:Al46; [4] Aissa et al., FASEB J. 1995, 9:A425; [5] Citro et al., FASEB J. 1995, 9:A392; [6] Senekowitsch et al., FASEB J. 1995, 9:A392; [7] Thomas et al., FASEB J. 1995, 9:A227. Supported in part by Bouygues SA, SAUR, Dolisos, LSH and Association Science Innovante.

Abstract
J. Allergy Clin. Immunol. 99:S175, 1997
TRANSATLANTIC TRANSFER OF DIGITIZED ANTIGEN SIGNAL BY TELEPHONE LINK.
J. Aïssa, P. Jurgens, W. Hsueh and J. Benveniste. Digital Biology Laboratory (DBL), 32 rue des Carnets, 92140 Clamart, France and NorthWestern University Medical School, Chicago, IL 60614, USA.
Ligands so dilute that no original molecule remained still retained biological activity which could be abolished by magnetic fields [1-3], suggesting the electromagnetic (EM) nature of the molecular signal. This was later confirmed by the electronic transfer to water (W) of molecular activity, either directly or after computer storage [4-7]. Here, we report its transfer via the telephone network. Ovalbumin (Ova) or W as control, was recorded (1 sec, 16 bits, 22 kHz) into a diskette in Chicago using a transducer and computer equipped with sound-card. Coded files were transferred to diskettes in DBL's computer as "attached documents" via Internet e-mail. After digital amplification they were replayed for 20 min via a transducer to 15 ml W-containing plastic tubes. Exposed W (dOva, dW), was then perfused to isolated hearts from Ova-immunized guinea-pigs. The heart operator was always blind while technical incidents revealed the identity of 4/19 files to the computer operator. Coronary flow variations were (%, mean+SEM, nb of measures): naive W (negative control), 4.9+0.3, 41; dW, 4.4+0.3, 58; dOva, 24.0+1.4, 30, p= 4.5 e -17 vs dW; Ova (0.1 µM, positive control), 28.9+3.7,19, ns vs dOva. The hitherto neglected physical nature of the molecular signal emerges: EM radiation under 22 kHz that can be digitized, transferred long distances and replayed to W, which then mimicks the activity of the source-molecule. This implies novel strategies in chemistry, biology and medicine. [1] Davenas et al., Nature. 1988, 333:816; [2] Benveniste et al., CR Acad Sci Paris. 1991, 312:461; [3] Benveniste et al., FASEB J. 1992, 6:A1610; [4,5] Aissa et al., FASEB J. 1993, 150:A146 & 1995, 9:A425; [6] Thomas et al., FASEB J. 1996, [7] Benveniste et al., FASEB J. 1996, 10:A1479. Supported by Association Science Innovante.

SPECIFICITY OF THE DIGITIZED MOLECULAR SIGNAL
J. Benveniste, J. Aïssa, P. Jurgens and W. Hsueh*
presented at Experimental Biology '98 (FASEB). San Francisco, April 20, 1998

Introduction
For several years, we have activated various biological systems using the electromagnetic (EM) signal of the agonist molecule instead of the molecule itself. Signals are applied electronically, in real time, to target cells or organs. Alternatively, they are digitally recorded on a computer, either directly, using a purpose-designed transducer, or via the public switched network, and then replayed [1-9]. One of the main questions in this complex process of detection, recording, transfer and replay, is the integrity of the applied signal, which is a prerequisite for the specific expression of the molecular signal. We therefore investigated the specificity of digitized EM signals of acetylcholine (d-ACh) and histamine (d-H).

Materials and Methods
Heart preparation.
Isolated hearts from male Hartley guinea-pigs, ~= 300 g, were perfused according to the Langendorff method using Krebs-Henseleit buffer (pH 7.4) gassed with O2/CO2, 95/5 %, at a pressure of 40 cm H2O at 37°C.
Recording.

ACh, or sodium acetate + choline chloride (AC) or H at 1µM, or water (W), were recorded (6 sec, 16 bits, 44 kHz) using a purpose- designed transducer and a computer equipped with a sound card.
Assays.

d-ACh and d-H were applied to isolated guinea-pig hearts, perfused or not with the ACh inhibitor atropine or the H1 receptor blocker, mepyramine, both at 1 µM. d-W or d-AC, and ACh or H (1 µM), were also applied as negative and positive controls respectively. Coronary flow was then measured every min for 30 min and changes were calculated as % maximal coronary flow variation compared to three "time 0" values. Student's t-test for unpaired variates (Plot 40, Sigma Plot) was used to assess statistical significance.
Mechanical parameters (min. and max. tension, heart rate) were observed and recorded using dedicated software.

RESULTS
The effects on the maximal variation in coronary flow are shown in Table 1. ACh, H (1 µM), d-ACh, d-H induced highly significant changes, whereas d-W or d-AC were indistinguishable from spontaneous flow variations.
Atropine inhibited both the effects of ACh and d-ACh, but not those of H and d-H. Mepyramine inhibited the effect of both H and d-H but not those of d-ACh.

Variations in coronary flow following application of d-ACh and d-H were highly significant compared with controls, d-W and d-AC.

A typical effect of d-ACh on heart frequency and its inhibition by atropine are shown (figure 1).
[Vasodilation was induced in guinea-pig skin by d-ACh, whereas d-AC was ineffective (figure 2).]